Sure. Visiting Jaisalmer, Udaipur and Ajmer is a splendid experience filled with a vibrant culture, stunning architecture, and rich history. Here are some travel tips:

Best Time to Visit:
The preferred time to visit Jaisalmer, Ajmer and Udaipur is between October and March when the temperature is pleasant.

Route and Accessibility:
All three cities are well-connected via rail, road, and air. Udaipur has an airport which is well-connected to major Indian cities. Jaisalmer is accessible by air (Jaisalmer Airport) and rail. Also, Ajmer is well connected by road and rail.

Altitude and Acclimatization:
All three places are at low altitudes so acclimatization is not an issue.

Accommodation and Booking:
It’s recommended to book your accommodation in advance. All three cities have a wide range of accommodations ranging from budget to luxury options. Udaipur and Jaisalmer also have unique heritage, and luxury palace hotels.

Clothing and Essentials:
Carry casual comfortable clothes. Considering Rajasthan's culture, it is advised to dress modestly. Sunscreen, hats, and sunglasses are important as the region is generally sunny. Comfortable shoes are necessary for exploring forts and palaces.

Health and Safety:
Carry a first aid kit and prescribed medicines if any. Stay hydrated as Rajasthan can get quite hot. Tap water is not always safe to drink so it’s better to buy bottled water.

Local Etiquette and Respect:
Respect local customs and traditions. Ask permission before taking photos of locals. Also, Hindi is the most spoken language but English is understood by locals involved in tourism.