Absolutely! Brno, Litomyšl, and Nelahozeves are all beautiful locations in the Czech Republic and each offer a unique travel experience. Here are my travel tips for you:

Best Time to Visit:
The best times to visit are in the late spring (May and June) when the weather is warm and all the flowers are in bloom, or during autumn (September and October) when the leaves are turning.

Route and Accessibility:
Fly into Prague, the capital of the Czech Republic, and Brno, Litomyšl, and Nelahozeves are all a few hours away by car or train. There are regular trains from Prague to Brno and Litomyšl. For visiting Nelahozeves, you might need to rent a car or take a bus as it is a small town.

Altitude and Acclimatization:
These cities do not require any particular acclimatization as they are not at high altitudes. However, remember to rest well after your flight to overcome any fatigue or jet lag.

Accommodation and Booking:
Book your accommodations in advance as these cities can be quite crowded, especially during the peak tourist season. Apartments, hotels, and guesthouses are available in all three cities.

Clothing and Essentials:
Bring comfortable clothing suitable for the weather. Carry a comfortable pair of walking shoes as these cities are best explored on foot. Don't forget to pack a sunhat, sunscreen, and sunglasses to protect yourself from the sun.

Health and Safety:
Follow standard travel safety guidelines. Keep copies of your important documents, avoid secluded areas at night, and always carry a map. Also, it’s a good idea to have travel insurance.

Local Etiquette and Respect:
Czech people appreciate if you greet them in their local language. A simple "Dobrý den" (Good day) can go a long way. It's respectful to tip at restaurants, 10-15% is standard. Always respect the local customs and traditions.