Absolutely! Here are some travel tips for your visit to Tokyo, Kyoto, and Osaka:

Best Time to Visit:
The best time to visit Japan is during spring (March to May) and fall (September to November) as you'll be able to witness the cherry blossom and the fall colors respectively. Winter can also be delightful with snow festivals and opportunities to ski.

Route and Accessibility:
Japan's public transportation system is highly efficient. Shinkansen (Bullet Train) is an excellent way to travel between Tokyo, Kyoto, and Osaka. You can use your Japan Rail Pass (JR Pass) for this. For local travels, metros, buses and bikes are also available.

Altitude and Acclimatization:
These cities are all at low altitudes so acclimatization is not typically an issue.

Accommodation and Booking:
Japan offers a range of accommodations from high-end hotels, traditional Ryokans to budget hostels. It is advisable to book your accommodations well in advance.

Clothing and Essentials:
Pack according to the season. If you're visiting during the cherry blossom season, carry a light jacket, as the weather can sometimes be cool. During summers, carry lightweight clothes with a hat and sunblock. Winters require warm clothing.

Health and Safety:
Japan is considerably safe. Tap water is drinkable, medical services are top notch. However, carrying a basic first aid kit is always recommendable.

Local Etiquette and Respect:
Japanese people are very polite and respectful. When visiting temples and shrines, follow the local customs, dress appropriately and respect the tradition. It's a common practice to bow when greeting. Also remember to take off your shoes when entering someone's house or certain traditional restaurants and shrines.