Definitely! Travelling to the beautiful cities of Prague, Brno, and Ostrava in the Czech Republic will definitely be an exhilarating experience. Here are some travel tips to assist you:

Best Time to Visit:
The most favourable time to visit these cities would be during the spring (April to June) and fall (September to October) when the weather is mild and the cities aren't crowded.

Route and Accessibility:
Upon arrival at Prague International Airport, you can opt for public transportation like trams, metro, or taxis. You could also consider renting a car for easier mobility. To travel from Prague to Brno and Ostrava, you can use the efficient Czech train system or inter-city buses.

Altitude and Acclimatization:
The altitude of these cities isn't high, so there's no need for specific acclimatization. However, it's always a good idea to take the first day of your trip easy to adjust to a new environment.

Accommodation and Booking:
The cities all have ample accommodation options ranging from luxury hotels to affordable hostels. It's advisable to book well in advance, especially if you are planning to visit during the peak tourist season.

Clothing and Essentials:
The temperature can significantly drop in the evening, so it would be a good idea to pack some warm clothing. Comfortable walking shoes are crucial if you intend to explore the cities by foot.

Health and Safety:
Ensure you have travel insurance. Pack any necessary prescription medications and a basic first aid kit. Always stay aware of your surroundings and keep your belongings secure.

Local Etiquette and Respect:
Czech people tend to be very polite, so be sure to respect their customs and traditions. It's good to learn a few basic Czech phrases. Dress respectfully, especially when visiting religious and revered places.