Hello! It's great to hear that you're planning a trip to the beautiful cities of Prague, Brno, and Ostrava in the Czech Republic. I'd be more than happy to provide you some travel tips.

Best Time to Visit:
The best time to visit these cities in the Czech Republic is during the spring months (April to June) and the fall months (September to October). During these periods, the weather is pleasant and the tourist crowds are relatively thinner.

Route and Accessibility:
Prague, being the capital, is well connected with most major international cities through direct flights. From Prague, you can take a train or bus to reach Brno and then continue onto Ostrava. All three cities have efficient public transportation systems including trams, buses and metro (only in Prague).

Accommodation and Booking:
There's a range of accommodations available in all these cities, from luxury hotels to budget hostels. It's recommended to book well in advance, especially if you're planning to visit during peak tourist seasons.

Clothing and Essentials:
Clothing largely depends on the time of your visit. In spring and fall, bring lightweight clothing but also a jacket for cooler evenings. If you're visiting in winter, pack warm clothing including thermal layers, a heavy jacket, and winter accessories. Don't forget comfortable shoes for walking around.

Health and Safety:
Tap water in the Czech Republic is safe to drink, and the country has a high standard of public health. However, travel insurance covering medical evacuation is highly recommended. Remember to keep emergency numbers handy.

Local Etiquette and Respect:
Czech people appreciate good manners - remember to say hello (Dobrý den) and goodbye (Na shledanou). It's also good practice to learn a few basic Czech phrases. Respect local customs and traditions, especially when visiting religious and cultural sites.