Sure, a trip to Ankara, Konya, and Aksaray promises a blend of history, culture, and stunning landscapes. Here are some recommendations:

Best Time to Visit:
The ideal time to visit these cities is in the spring (April to June) and autumn (September to November) when the weather is temperate.

Route and Accessibility:
You can start your journey in Ankara, the capital city of Turkey, and then travel to Konya by train or bus. From Konya, travel to Aksaray by bus.

Altitude and Acclimatization:
These locations are not situated at significantly high altitudes, so acclimatization isn't a major concern. However, do let your body adjust to the new climate and time zone.

Accommodation and Booking:
Book your accommodations in advance. All three cities have a variety of options depending on your budget, including luxury hotels, budget guesthouses and hostels.

Clothing and Essentials:
Pack according to the season. Summers can be hot, so lightweight clothing, sunglasses, and sunscreen are a must. During the colder months, consider taking warm clothing. Comfortable shoes are a must for exploring the cities.

Health and Safety:
Ensure your travel insurance covers medical expenses. Keep an emergency contact list, and carry a first-aid kit and any prescribed medications.

Local Etiquette and Respect:
Turkish people are known for their hospitality. Always remember to respect the local customs and traditions. When visiting religious places, dress modestly, and remember to remove your shoes when entering mosques. In Konya, this would be particularly relevant when visiting the Mevlana Museum, which is the mausoleum of Rumi, a Sufi mystic.