Absolutely! Italy is a fantastic country and the cities of Rome, Venice, and Florence offer incredible beauty and history. Here are some travel tips:

Best Time to Visit:
The best time to visit these Italian cities is during the spring (April to June) and fall (September and October) when the weather is pleasant and there are fewer tourists.

Route and Accessibility:
The most convenient way to travel between these cities is by train. Italy has an extensive and efficient rail network. From Rome, you can take a direct train to both Venice and Florence.

Altitude and Acclimatization:
These cities are all at sea level or close to it, so altitude is not a concern.

Accommodation and Booking:
Booking your accommodation in advance is advisable, particularly during the peak tourist season. All three cities offer a broad range of accommodation options from budget to luxury.

Clothing and Essentials:
Comfortable walking shoes are a must as all three cities are best explored on foot. Summers can be hot, so light, breathable clothing, a hat, and sunblock are advised. Winters can be cool and wet, especially in Venice, so pack accordingly.

Health and Safety:
Italy is generally safe but do be cautious of pickpockets in crowded tourist locations. In terms of health, maintain regular hygiene practices and drink bottled water if you're unsure of the tap water.

Local Etiquette and Respect:
Italians appreciate politeness and courtesy. It's expected to say 'please' and 'thank you' in interactions. When visiting churches, dress appropriately - shoulders and knees should be covered. Stand-up cafes are cheaper than sit-down ones, don't be surprised if the prices change based on where you're served!