Sure, I'd be happy to provide you with some travel tips for your trip to Panama.

Best Time to Visit:
The best time to visit Panama City, Colón, and David is during the dry season, which falls between mid-December to mid-April. During this period, sunny skies and balmy temperatures make sightseeing and outdoor activities enjoyable.

Route and Accessibility:
The most convenient way to travel around Panama is by airplane or bus. You can easily get flights from Panama City’s Tocumen International Airport to major cities. Buses are also frequent and relatively inexpensive.

Altitude and Acclimatization:
Panama's altitude is not very high with respect to sea level so you don't have to worry about altitude sickness. Just make sure to keep yourself hydrated and put on sunscreen during the day.

Accommodation and Booking:
Both budget and luxury accommodations can be found in all major cities. It is advised to book your stay in advance to avoid any inconvenience.

Clothing and Essentials:
As Panama has a tropical maritime climate, lightweight clothes are perfect for the day. Also remember to carry your swimwear, hat, sunglasses, and a good sunscreen. Additionally, pack a strong insect repellent and a travel raincoat in case of sudden downpours.

Health and Safety:
Avoid tap water and stick to bottled water to avoid any health issues. Also ensure your routine vaccines are up-to-added before the trip. For safety, stay vigilant in crowded areas and don't flaunt valuables.

Local Etiquette and Respect:
Polite behavior is highly valued in Panama. Also respect the local culture and traditions. Understand that 'manana' (tomorrow) often means 'sometime in the future', so be patient. When visiting indigenous communities, it's respectful to ask for permission before taking photos.

Enjoy your trip to Panama. It's a beautiful country with much to offer.