Absolutely! Travelling across the beautiful cities of Prague, Brno, and Ostrava in Czech Republic will surely be an exciting experience. Here are some travel tips to help you along the way:

Best Time to Visit:
The best time to visit Czech Republic is generally the spring (April-June) and fall (September to early November) when the weather is mild and pleasant.

Route and Accessibility:
These cities are well connected by the Czech Republic's efficient rail and road system, and it is easy to travel from one to another. From Prague, you can take direct trains to Brno and then onwards to Ostrava.

Altitude and Acclimatization:
The cities of Prague, Brno and Ostrava are not situated at high altitudes, so you do not need to worry about acclimatization.

Accommodation and Booking:
Book accommodations in advance. All three cities offer a range of accommodations options from luxury hotels to budget hostels.

Clothing and Essentials:
Prague, Brno and Ostrava have moderate climates, but it can get a bit chilly in the evenings and early mornings. Pack light clothing for the day and couple of jackets for the night. Make sure to add an umbrella or a raincoat to your packing list as unexpected showers can happen.

Health and Safety:
Carry travel insurance, eat healthily, and drink plenty of water. European Health Insurance Card holders can access state provided healthcare in Czech Republic.

Local Etiquette and Respect:
While in Czech Republic, observe basic etiquette. Learn a few words in Czech, it could help break the ice with locals. Czechs generally appreciate quiet and respect, so avoid being overly loud in public places.

Enjoy your journey and make lots of lovely memories!