Sure! Tokyo, Kyoto, and Osaka are vibrant cities in Japan full of history and culture. Here are some travel tips for your trip:

Best Time to Visit:
The ideal time to visit these cities is during spring (March to May) when cherry blossoms are in bloom, and autumn (September to November) when leaves change color. Also, they generally have mild, pleasant weather.

Route and Accessibility:
You can reach Tokyo directly via flights. For domestic travel in Japan, consider a Japan Rail (JR) Pass which can save you money on Shinkansen (bullet trains) that connect Tokyo, Kyoto, and Osaka.

Altitude and Acclimatization:
These cities are at low altitude so you won't need to worry about acclimatization.

Accommodation and Booking:
It's recommended to book your hotels in advance due to high demand, especially during cherry blossom and autumn foliage seasons. There are various types of accommodations available, from luxury hotels to traditional Japanese inns (Ryokan) and budget-friendly hostels.

Clothing and Essentials:
Pack according to the season you're traveling. A good pair of walking shoes is essential as there will be lots of walking especially if you plan to visit temples, markets, and museums. Don't forget your travel plug adapter for Japan's Type A or B sockets.

Health and Safety:
Japan is generally safe and clean but it's always good to have travel insurance. If you have any dietary restrictions, have a translated card explaining them because Japanese cuisine extensively uses seafood and pork.

Local Etiquette and Respect:
Japanese people are known for their politeness. Learn some basic etiquette like bowing when you meet someone and saying 'itadakimasu' before meals. Be quiet on public transportation, and always keep to the left on escalators, except in the Kansai region where Osaka is, where people keep to the right.
In Kyoto, be respectful when visiting temples and refrain from touching Geishas or Maikos.