Sure, all the places mentioned by you are famous, cultural spots in Malta and Italy; here are some travel tips for a smooth trip:

Best Time to Visit:
Generally, the best time to visit these Mediterranean cities is from April to June or from September to October when the temperature is pleasant. The peak tourist season is during the summer months of July and August, but it can be crowded and hot.

Route and Accessibility:
These cities are well-connected by flights and ferry services. Internal transport includes buses, taxis, and in some places, horse-drawn carriages (such as in Mdina).

Altitude and Acclimatization:
These cities are located at sea level, so you won't need to acclimatize. However, do remember to relax and catch up on your sleep to counter the jet lag if you're coming from far away.

Accommodation and Booking:
It’s advisable to book your accommodations in advance, especially during the peak tourist season. Accommodation ranges from premium hotels to budget-hostels, so select as per your preference and budget.

Clothing and Essentials:
Light, breathable clothing is advisable for the hot summer months. Carry sunscreen, a hat, and sunglasses. For the cooler months, a light jacket will suffice. Don't forget comfortable footwear as these cities are best explored on foot.

Health and Safety:
Drink plenty of water to stay hydrated. European Global Health Insurance Card (EHIC) holders are entitled to free emergency medical care.

Local Etiquette and Respect:
Italians and Maltese appreciate politeness, so remember to say please (per favore in Italian, jekk joghgbok in Maltese) and thank you (grazie in Italian, grazzi in Maltese). Churches are places of worship so maintain decorum. Wear modest clothes while visiting religious spaces.

Enjoy your trip and experience the rich history of these beautiful cities!