Sure, I'd be glad to assist. Visiting Samara, Kazan, and Cheboksary offers a unique take on Russian culture and history. Here are some travel tips for these cities:

Best Time to Visit:
The best time to visit these cities would be between late May and early September when the weather is the most comfortable. Do note July and August can be a little crowded due to summer vacations.

Route and Accessibility:
All these cities are well-connected by train and flight from Moscow. Kazan is approximately an 11 hours train ride from Moscow, Samara is about 15 hours, and Cheboksary is roughly 12 hours. It's easier to fly to Samara and Kazan but, Cheboksary is mainly accessible by train or bus.

Altitude and Acclimatization:
These cities are not situated at high altitudes, so acclimatization won't be an issue.

Accommodation and Booking:
Each city offers a variety of accommodations ranging from luxury hotels to budget hostels. It is advisable to pre-book, especially during the peak tourist seasons.

Clothing and Essentials:
If you're visiting in the summer, typical city wear is suitable, but do carry an umbrella and a light jacket for sudden showers. Winter can be extremely cold so bundle up if you're planning a visit then.

Health and Safety:
Russian cities are usually quite safe, but it's always a good practice to be aware of your surroundings and keep an eye on personal belongings.

Local Etiquette and Respect:
As in any other Russian city, it's important to respect the local traditions and customs. Russians greet each other with a firm handshake and it is common to offer and accept small gifts when visiting someone's home. Also, remember to remove your shoes when entering a Russian house.

Remember to have cash on hand as credit cards are not accepted everywhere, and always carry your passport - Russian police have the authority to stop people and ask for documentation.

Also, learning a few phrases in Russian could be useful.